The Koopalings: Bowser's evil children that each take over a certain main galaxy to slow down Mario and Co. They consist of Larry, Morton, Wendy, Iggy, Roy, Lemmy and Ludwig. (Terrain Rock, Thwump Tower, Boo Rock, Clouded Calamity, Warm Core, Cannon Canyon, Gray Matter Ship)

Bowser Jr.: Bowser's youngest son even below the koopalings and Bowser's pride and joy. You meet him several times through out the game. In places such as Splish Splash Galaxy and Castle Grandé Galaxy. (Splish Splash, Castle Grandé, Shell-Bell Beach, Dry Boned, Black Hole, The Galactic Empire)

Kamek: Bowser's evil henchman wizard, who has enough power to bring robots to life and to make people invisible and so on. You will meet Kamek in several instances in the game such as in Ellipse Eclipse Galaxy where he is huge, and Blizzard Wizard Galaxy where he uses frosty mist to freeze the player, (Blizzard Wizard, Ellipse Eclipse, Black Hole, The Galactic Empire)

Potty the Piranha Nub: A small piranha nub who was magically enhanced by Kamek, turning him into a giant uncontrollable path of destruction. Potty's one weakness is his Yoshi like tongue which needs to be stepped on 3 times. (Rainbow Egg)

G. Troopa the Giant Koopa: A scientist who aided Mario and Co. for a while, until Kamek stopped the helping hand by turning him giant and evil. G. Troopa's battle slightly resembles that of Hookbill's from Yoshi's Island. Simply use a yoshi to throw three bombs into the stomach of G. Troopa then jump on him stomach once he is flat on his back. (Koopa Paratroopa)

Biggest Boo: A giant boo that is bigger than King Boo yet he is not the king. Nor is he afraid of people looking at him. He will charge at you at which time you will have to use Yoshi and the bulb berry to shine some light on the face of the boo to blind him then use a bomb boo to sling it at the back side of Biggest Boo. If this is done 3 times you will be rewarded a star and the boo will disappear. (Boo Bleck)

Gumdrop Mole: An evil miniboss mole, covered in candy and appears pink, in the Cookie Confection galaxy. He sits upon a very large stack of cookies while he throws explosive gumdrops at you. Occasionally a mushroom or coin or a green gumdrop. The green ones don't explode immediately but if you don't it will. Three of these to Gumdrop's noggin should do the trick. (Cookie Confection)

Flakey the Confused: A large snow covered Tap-Tap from the mountainous regions of Snowflake Lake. He is confused because his eyes are always in swirls and he never is sure of where he is going. Simply lead him into a pit of fire and melt him. Depending on how long it takes for him to melt depends on the force and strength of your fire, made by your fire flower. (Snowflake Lake)

King Lakitu: The lakitu king that lives in the clouds of space. He will be made large by Kamek and then the battle will start. He will toss down many spinies at one time, up to 5. Use a Super Leaf to run around in circles on the ground to make the king dizzy and the spinies. Pick up a spiny and throw it at the king. After the 2nd hit he will start shooting lightning. Three sharp spinies will kill the king. (Shrouded Cloud) 

Senior Calor: The father of King Caliente. He lives in a waterfall of lava that pours into a hole covered in ice. His tentacles are very large and he will try to crush you with them. He will spew lava from his snout and fire lava asteroids. Using an ice flower freeze each of his tentacles once they are on the ground then ride up one and freeze his head, once done his heart will be exposed and using a hammer hit it. Once done three times Senior Calor will be finished. (Icy Spicy)

Mecha Bowser Jr.: A mechanical larger version of the Koopa Prince himself. His operating system lies deep withing the robot. You will be given a time limit, 4 minutes, to go find the entrance to the robot and go through the deadly mecha maze to find the self destruct button. If not the robot will go off the edge of the planet and you will too.

GummyDrop: The sticky mole that dwells in the caverns of the Choconilla Planet. He is brown and covered with chocolate. His tactics are similar to those of Gumdrop. Except he pops out of different holes from the ground to make the battle harder. Plus the chocoalte ceiling is dripping huge chunks of chocolate, so avoid them at all costs. (Choconilla)

Spencer Spiny the Sharp: Is a spiny that lived in an underground tunnel and burrows as though he were a mole. However, Kamek once again arrived on the scene and maximized Spencer's size. Spencer now burrows huge tunnels weaving through each other. What you need to do is grab a boulder laying around and when you have gotten behind Spencer throw it at his behind. Quickly run for safety. Do this a few times and then he will become very fast and hard to hit. (Plunder Under)

Boney the Huge: An ancient Dry Bones statue that had sank in the sand centuries ago. Kamek has come to revisit the old and make it anew. He made Boney come back to life and had him rampaging through the sand. What you needed to do was grab an E.Wand and stun Boney then, grab a hammer to smash Boney and have him collapse then use an ice flower to kill the flare in his eyes. Once the fire was completely put out from this ancient beast it would go back to being a statue deep in the sand. (Dry Boned)

Bowser Koopa: The evil King of the Koopas who has been bested in the past but not this time. You will fight him once in the Black Hole Galaxy, where you simply have to pull a lever to release him into a black hole. The second battle takes place in the Galactic Empire, where Bowser has aquired a Clown Car, here you must use every item to help you defeat the evil Bowser. The third battle takes place after Bowser has gotten the Purest Power, in the Mushroom Kingdom. You must use Rosalina's wand to fire powerful magic blasts into the mouth of Bowser. Once this is done 5 times you will have won the game. However, keep on the look out for the koopalings they help by acting as regular enemies through out the final battle. They will try to steal Rosalina's wand and give it to Bowser, one simple jump to the head should do the trick. However, in some cases like Iggy and Morton, where they are very large or very tall it could be difficult to move forward. (Black Hole, Galactic Empire, The Mushroom Kingdom)