(P)= Playable character. The rest without, are unplayable on the Adventure part.
(p)= Minor playable character. Play as them on the Starship and in certain mini-games.
(P)Mario: An Italian plumber that has brought his friends to help him save the universe from the evil Bowser and the Koopalings.
(P)Luigi: Mario's younger brother helping him save the universe.
(P)Princess Peach: Mario's best friend and supposed girlfriend from the Mushroom Kingdom, helping him save the universe.
(P)Toad: Mario's other friend, who is more than excited to go on another world saving adventure.
(p)Professor Elvin Gadd: The kooky scientist who has invented and furnished some new gadgets and gismos for Mario and co.
Lumua: Rosalina's message Luma sent by Rosalina to help aid Mario and Co. save the universe.
Rosalina: The princess of the Cosmos who stayed in the Mushroom Kingdom to watch over the lands while Peach was away, guarding it so Bowser does not attempt to steal it.
Bowser: The evil King of the Koopas who once again is out for more than just the princess.
Bowser Jr.: The evil 8th son of the King of the Koopas, helping cause chaos across the universe is his life.
The Koopalings: The evil children of the King of Koopas, who took over certain galaxies in the universe.
Kamek: The koopa wizard that helped Bowser and the Koopalings become more powerful to destroy the Mario group.
(p)Toadette: Toad's best friend who decided to join journey but not participate in the action. instead she sits on the ship and offers advice and items to the players.
(p)Jaque the Friendly Lakitu: A young hip hip friend of Bo-Bum who loves nothing more than exploring.
Bo-Bum the Bob-bomb: A quiet and old bob-omb with tales of the old Galaxies.
(p)Yoshi: A variety are spread all across the universe for help. Galaxies that allow Yoshi's can be unlocked on the Starship secret room.
The Kings and Queens of the Mushroom Kingdom: They will contact you via E.Gaddevision once a wand has been recovered.
